人源性神经营养素-6对体外培养海马神经元存活的促进作用

Promotive Effect of Human-derived Neurotrophin-6 on the Survival of Cultured Hippocampal Neurons

分离出一周SD乳鼠海马组织,进行离体海马细胞培养;在培养基中加入神经营养素-6成熟肽片段,通过神经元特异性烯醇化酶免疫组化染色,计数存活海马神经元数量,与对照组比较,观察神经营养素-6对神经元存活的促进作用。结果显示,神经营养素-6实验组海马神经元存活数目显著高于对照组。表明人源性神经营养素-6可以促进体外培养神经元的存活。

The hippocampus of P7 SD rat was isolated. The hippocampal neurons were cultured with the culture medium added mature peptide of human-derived neurotrophin-6. The surviving hippocampal neurons were counted at different time of culture after neuron-specific enolase immunohistochemical staining. It was shown that the number of surviving hippocampal neurons in the experimental group of neurotrophin-6 was significantly increased. This study indicated that the human-derived neurotrophin-6 could enhance the survival of the cultured neurons, suggesting its nutritious and protective action on the nervous system.