摘要
目的 探讨p2 7基因转染对血管损伤后新生内膜形成的影响。方法 以腺病毒为载体转染p2 7基因 ,用MTT法观察其对血管平滑肌细胞增殖的影响 ,用流式细胞仪检测细胞周期 ;在兔颈总动脉球囊损伤模型上转染p2 7基因 ,采用免疫组织化学法检测p2 7基因的表达 ,检测其对新生内膜形成的影响。结果 p2 7可明显抑制培养的血管平滑肌细胞增殖 ,细胞停滞于G1期。兔颈总动脉球囊损伤后转染p2 7基因可以减少新生内膜的形成达 2 7 4 3%。结论 以腺病毒为载体转染p2 7基因可以有效地抑制血管平滑肌细胞增殖及血管损伤后新生内膜的形成。
Objective To evaluate the function of p27 gene on the neointimal formation after balloon injury in rabbit carotid artery. Methods The cultured vascular smooth muscle cells (VSMCs) were infected with Adp27. Using MTT assay we measured the proliferation of VSMCs. Balloon injured rabbit carotid arteries were also infected with Adp27, and then we evaluated the ratio of intima to media. Results MTT assay of rabbit VSMCs proliferation demonstrated an obvious inhibition in Adp27 treated group. And p27 overexpression had an effect on the cell cycle. It could induce cell cycle arrest in G1 phase and reduce the cells in S phase. Overexpression of p27 could also reduce the neointimal hyperplasia by 27.43%. Conclusion The p27 gene can reduce the neointimal formation after balloon injury in rabbit carotid artery effectively.
出处
《中国介入心脏病学杂志》
2004年第5期305-308,共4页
Chinese Journal of Interventional Cardiology
基金
北京市自然科学基金资助(7032027)
教育部教育振兴行动计划特殊专项(九八五工程)资助
