深低温冷藏骨组织存活时限的研究

The study on the time limit of viability of cryopreserved bone tissue

目的 研究储藏在液氮中的骨组织保持存活状态的时限 ,为吻合血管同种异体骨移植中供体的供给时机提供理论依据。方法 对冷藏不同时段的骨组织进行光镜、电镜及琥珀酸脱氢酶 (SDH)染色检查。结果 冷藏 4个月后的骨组织结构形态学正常 ,骨细胞SDH染色阳性 ,而冷藏 6个月后的骨组织有线粒体肿胀的超微结构改变 ,骨细胞SDH染色阴性。结论 存储于液氮中的骨组织维持存活状态的最佳时限为 4个月。

Objective To study the time limit of viability of bone tissue stored in liquid nitrogen and provide the theoretical basis for the time when vascularized allograft bone transplanted into receptor.Methods Specimens of cryopreserved bone tissue in different storage periods were analyzed under light and electron microscope and succinic acid dehydrogenase (SDH) stain.Results The morphology of bone tissues appeared well and bone cells were stained by SDH in specimens with storage period of 4 months,while swollen mitochondria were detected by electron microscope and bone cells were not stained by SDH in specimens with storage periods of 6 months.Conclusion The optimum time limit to viability of bone tissue preserved in liquid nitrogen was 4 months.