榄香烯抑制脑胶质瘤细胞增殖的比较研究

A comparison study of the proliferation inhibitory effect of elemene on glioma cell

目的 比较研究榄香烯对胶质瘤细胞和正常神经胶质细胞的不同作用效果。方法 采用噻唑蓝 (MTT)方法检测榄香烯体外抑制大鼠C6胶质瘤细胞增殖的有效浓度 ;原代培养SD大鼠星形胶质细胞 ,比较观察不同浓度榄香烯对于C6胶质瘤细胞和原代星形胶质细胞的作用效果差异。结果 榄香烯能显著抑制C6细胞增殖 ,IC50 为 19.73～ 3 9.75mg/L。体外比较观察 ,榄香烯作用浓度在 80～ 10 0mg/L以内 ,不会引起正常星形胶质细胞的死亡和形态学的改变。然而 ,当药物浓度达 3 0～ 40mg/L ,C6胶质瘤细胞大部分发生了脱壁和死亡。 结论 榄香烯的抗肿瘤增殖作用具有一定的特异性。

Objective To evaluate the proliferation inhibitory effect of elemene on glioma cell and primary astrocyte.Methods The effects of elemene on cell proliferation were studied in vitro by using MTT assay;Astrocyte-enriched culture from postnatal rat cerebral cortex tissue was prepared.The cytocidal effects of elemene in the presence of various concentrations were measured.Through assessing the histological effect,the effect of elemene on astrocyte and the glioma cell was compared.Results Elemene exhibited a marked antiproliferative effect on rat glioma cell C6.The IC 50 was about 19.73-39.75 mg/L;Also the results showed 80-100 mg/L elemene did not affect the astrocyte.In glioma cells,addition of 30-40 mg/L elemene resulted in a marked growth suppression.Conclusion The antitumor effect of elemene is specific.