外加磁场对半乳糖化白蛋白磁性阿霉素纳米粒在大鼠体内分布的影响

Study on effect of magnetic field application on targeting distribution of Gal-HSA magnetic nanoparticles containing adriamycin

目的 观察半乳糖化白蛋白磁性阿霉素纳米粒在正常肝脏中的靶向性 ,并观察半乳糖化白蛋白磁性阿霉素纳米粒在全身各脏器的分布特征及外加磁场对其分布的影响。方法 大鼠正中开腹 ,肝动脉插管并固定 ,肝动脉注射12 5I半乳糖化白蛋白磁性阿霉素纳米粒 (相当于阿霉素0 .5mg/kg) ,左外叶加磁场 ,磁场应用 3 0min ,移去磁场后 ,动物立即处死 ;对照组 :肝动脉注射半乳糖化白蛋白磁性阿霉素纳米粒 ,左外叶不加磁场 ,3 0min后 ,移去磁场后 ,动物立即处死 ,立即取靶区肝、非靶区肝、肾、心、肺、小肠、脾及周围血作γ计数。肝组织作病理切片。结果 注入的纳米粒75 %～ 85 %分布于肝脏 ,其他脏器极少。病理切片显示磁区小动脉见大量纳米粒存在 ,对照组及非磁区肝中纳米粒很少见。结论 半乳糖化白蛋白磁性阿霉素纳米粒在正常肝组织中有明显的磁靶向性 ;在磁场作用下 ,半乳糖化白蛋白磁性阿霉素纳米粒主要分布于肝脏 ,其他脏器含量很少 ;实验组肾、心、肺、小肠、脾及外周血与对照组的放射活性比较明显降低 。

Objective To observe the hepatic targeting of Gal-HSA magnetic nanoparticles containing adriamycin and its effect of magnetic field application on targeting distribution of in vivo,and probe into distributed characterization in the normal rat organs.Methods Abdominal exposure was carried out through a midline abdominal incision.A cannula was inserted into the hepatic artery and mounted.Gal-HSA magnetic nanoparticles containing adriamycin were inject into hepatic artery (equivalent dose of free adriamycin 0.5 mg/kg).After the left lateral lobe of the liver was exposed to the magnetic field for 30 min,the rat was immediately killed.Administration of equal close of nanoparticles in the absence of magnetic field served as a control.The targeting liver tissue,non-targeting liver,kindey,heart,lung,small intestine,spleen and blood was analyzed for 125 I-counts.Liver tissue was examined for histology.Results 75%-85% of injected radioactivity dose distributed in liver.The histology examination demonstrated a lot of nanoparticles accumulating in arteriola.In control group,no accumulation of nanoparticles was found,only some single nanoparticles dispersed in hepatic sinus.Conclusion Gal-HSA magnetic nanoparticles containing adriamycin in normal rat liver had remarkable hepatic targeting and good magnetic targeting.The Gal-HSA magnetic nanoparticles containing adriamycin injected mainly distributed in liver.The radioactive ratio of heart,kidney,spleen,small intestine and blood in experiment group was descreased significantly as compared to control,suggesting the relative exposure of these organs in experimental group was reduced as compared to control.