摘要
为寻找更多小麦株高的QTL ,并用于品种改良和分子标记辅助育种 ,利用江苏地方品种望水白与墨西哥小麦品种Alondra杂交构建的重组自交系群体 (10 4个家系 )在 3个试验环境 [1997年和 1999年于江苏省农业科学院 (以下简称农科院 )、2 0 0 2年于南京江宁试验区 (以下简称江宁 ) ]中的株高资料 ,进行了株高性状的QTL分析。共检测到 4个影响小麦株高的QTL ,它们分别位于 1D、2B、4A和 4D染色体上 ,其中位于 1D染色体上的QTL来自地方品种望水白 ,其余 3个QTL均来自矮杆亲本Alondra;单个QTL能够解释 10 3%~ 33 8%的表型变异 ,降低株高效应为 3 2~ 7 4cm ,每个环境条件下检测到的所有QTL能解释 35 0 %~ 4 4 5 %的表型变异 ,4A和 4D染色体上的QTL在 3个试验环境下均能被检测出来 ,说明这 2个QTL可以用于品种改良和标记辅助育种 ;4D染色体上的QTL可能是矮杆基因Rht
To find more QTLs for plant height in wheat,and apply them in the varietal improvement and marker-assisted breeding,a population of 104 recombinant inbred lines (RILs) derived from the cross of Wangshuibai (a Jiangsu landrace) and Alondra (Mexico variety) was used to detected the QTLs for plant height.The individuals of the population and their parents were assessed in the field trials at two locations in 1997,1999 and 2002.With the methods of interval mapping and MQM mapping,four putative QTLs for plant height were detected on chromosomes 1D,2B,4A and 4D,respectively.The QTL on chromosome 1D came from Wangshuibai,and the other QTLs from Alondra.Each QTL was able to explain phenotypic variations by 10.3%—33.8% and decrease the plant height from 3.2—7.4 cm.In each environment the total putative QTLs explained about 35.0%—44.5% of the total phenotypic variation.The QTLs on chromosome 4A and 4D were detected in the three years and should be useful in future marker-assisted-selection programs.The QTL on chromosome 4D was probably a dwarf gene Rht-D1b.
出处
《江苏农业学报》
CSCD
2004年第4期201-206,共6页
Jiangsu Journal of Agricultural Sciences
基金
国家"8 63"项目 ( 2 0 0 1AA2 110 2 1)
( 2 0 0 2AA2 0 70 0 3 )
江苏省高新技术研究项目 (BG2 0 0 2 3 0 4)