摘要
水牛免疫乳提取物中的免疫球蛋白(IgG)以终浓度为1.0mg/ml加入到霍乱弧菌和伤寒杆菌的培养液中时,能有效地抑制这两种菌的生长;IgG在pH为4.0～9.0范围比较稳定,在pH低于4.0条件下,随着pH下降,变性率剧烈上升;pH为2.0～4.0时,IgG的活性随着胃蛋白酶用量和作用时间的增加而下降,pH2.0时下降速度最快;pH为7.4时,胰蛋白酶对IgG活性影响很小,不改变IgG的凝集价。
Bacteriostasis effects to vibrio cholera and to Bacillus typhoid fever could be observed when the immune-globulin(IgG)abstracted from buffalo immune mi lk was in the cultivation liquid of these two types of bacteria with the ultimat e concentration of l.0mg/ml.Such IgG was relatively stable with the range of pH4 .9～9.0.When pH value below4.0,the denaturation rate of IgG went up sharply with the decrease of pH value.When pH value ranged from 2.0 to 4.0,IgG activity went down with the increase of pepsin dosage and action time on it,and the fastest d ecline had been found with pH2.0.In addition,when pH value reached 7.4,trypsi-na se had minute influence on IgG activty and the antibody titer remained unchanged .
出处
《中国奶牛》
北大核心
2004年第6期18-21,共4页
China Dairy Cattle
基金
广西自然科学基金资助项目(桂科自20007010)
