CEA-mRNA和CA19-9对恶性腹水的诊断价值

The Values of CEA-mRNA and CA19-9 in Diagnosing Malignant Ascites

目的 :分析 CEA- m RNA和 CA19- 9指标在诊断与鉴别诊断恶性腹水方面的应用价值。方法 :采集腹水为检测标本 ,其中恶性腹水、良性腹水各 32份。应用反转录套式聚合酶链反应技术检测 CEA- m RNA、磁分离酶联免疫测定技术方法检测 CA19- 9。结果 :良性腹水组 ,CEA- m RNA和 CA19- 9阳性率分别为 6 .2 % (2 / 32 )和 0。恶性腹水组 ,CEA-m RNA和 CA19- 9阳性率分别为 78.1% (2 5 / 32 )和 5 9.4 % (19/ 32 ) ,特异性分别为 93.8%和 10 0 % ,两者联检的阳性率为87.5 % (2 8/ 32 )。恶性腹水组与良性腹水组检测结果的差别 ,均具有显著性 (P<0 .0 1)。结论 :在诊断和鉴别诊断恶性腹水方面 ,CEA- m RNA和 CA19- 9均具有较高的特异性和敏感性 ,具有较高的应用价值 ;两者联检 ,可以进一步提高检测敏感性。

Objective:To analyse the values of CEA-mRNA and CA19-9 in diagnosis and differential diagnosis of malignant or benign a scites.Methods:There were 32 malignant ascites and 32 benign ascites in the st udy.CEA-mRNA was detected in ascites by reverse transcriptase nested primers po lymerase chain reaction and CA19-9 in ascites by magnetic antibody immunoenzymetric assay.Results:In the benign ascites group, the positive rate of CEA -mRNA was 6.2% (2/32) and CA19-9 was 0. In the malignant ascites group, the po sitive rate of CEA-mRNA was 78.1% (25/32) and CA19-9 was 59.4% (19/32), the sp e cificity of CEA-mRNA was 93.8% and CA19-9 was 100%, the positive rate was 87.5 % (28/32) when combined detection was performed.The positive results of CEA-mRNA and CA19-9 were significant between the malignant group and the benign group (P<0.01).Conclusion:CEA-mRNA and C A19-9 were useful indexes in diagnosis and differential diagnosis of malignant asc ites. Combined detection could improve the sensitivity.