电针对消化道肿瘤患者围手术及围化疗期T淋巴细胞亚群和淋巴细胞转化功能的影响

Effects of Electroacupuncture on Peripheral T-lymphocyte Subsets and Proliferation in Patients with Gastrointestinal Tumor Treated with Surgery and Chemotherapy

目的 观察电针对消化道肿瘤患者围手术及围化疗期 T淋巴细胞亚群和淋巴细胞转化功能的影响。方法 应用流式细胞仪测定消化道肿瘤患者手术或手术加化疗及针刺治疗前后 T淋巴细胞亚群的变化 ,同时用 MTT法检测外周血淋巴细胞转化功能。结果 手术后第 3日 ,患者 CD3+ 细胞百分率、淋巴细胞转化功能明显低于手术前 (P<0 .0 5 ,P<0 .0 1) ,而电针治疗组患者 CD3+细胞百分率、淋巴细胞转化功能则未见降低 ,与手术组病人相比有显著差异 (P<0 .0 5 )。手术后第 10日 ,患者淋巴细胞转化功能基本恢复至手术前水平 ,电针组患者 CD8+ 细胞明显减少 (P <0 .0 5 ) ,CD4 + / CD8+ 比值明显升高 (P <0 .0 5 )。手术并化疗后第 3日 ,CD3+细胞百分率 ,CD4 + / CD8+比值较手术前有降低 ,但统计学上无差异 ,电针能明显提高手术加化疗后第 3日患者的 CD4 + 细胞百分率 (P<0 .0 5 )以及 CD4 + / CD8+ 比值 (P<0 .0 5 ) ,与手术前自身比较 ,电针治疗能提高手术并化疗后患者的淋巴细胞转化功能 (P<0 .0 1,P<0 .0 0 1)。

Objective To explore the effects of electroacupuncture(EA) on peripheral T-lymphocyte subsets and proliferation in patients with gastrointestinal tumor treated with surgery and chemotherapy. Method The changes in peripheral T-lymphocyte subsets were evaluated by flow cytometry, peripheral lymphocyte proliferation was detected by MTT assay. Results Results The results indicated that the CD3^+ T cells and lymphocyte proliferation decreased significantly on day 3 post-operation (P<0.05, P<0.01, respectively), whereas no changes were observed in EA treatment group. Ten days post-operation, the lymphocyte proliferation back to the level of pre-operation. At the same time, EA treatment decreased CD8^+ T cells (P<0.05) and increased the CD4^+/CD8^+ ratio on day 10 post-operation(P<0.05). In the other groups which patients undergone surgery and chemotherapy, CD3^+ T cells ,CD4^+/CD8^+ ratio declined on day 3 post-operation combined chemotherapy, EA could increase CD4^+ T cells(P<0.05)and CD4^+/CD8^+ ratio(P<0.05) in patients who suffered surgical procedure and chemotherapy and enhanced the lymphocyte proliferation when compared with pre-operation (P <0.001). Conclusion EA could improve the immune function in patients with gastrointestinal tumor undergone surgery and chemotherapy.