新型高效液相色谱激光诱导荧光检测器的研制

Design of Laser-Induced Fluorescence Detector for High Performance Liquid Chromatography

采用共聚焦结构,研制了一种高效液相色谱激光诱导荧光检测器(HPLC LIFD)。采用473nm的半导体泵浦固体激光器为光源,设计了一种专用于共聚焦的HPLC LIFD检测池。该池的物理体积为7μL,有效体积约为0 1nL,入口通道与出口通道的夹角为120°,无直角区和变径区,无滞留和积累气泡的死角,对谱带展宽影响小。以核黄素标准品为测试物评价了该体系并利用该系统测定了实际样品中核黄素的含量。

Based on confocal configuration, a laser-induced fluorescence detector (LIFD) for high performance liquid chromatography (HPLC) is described and evaluated. A diode double-pumped solid-state laser emitting at 473 nm is used as excitation source. A detection cell of 7 μL physical volume and 0.1 nL effective optical volume was designed for conventional HPLC-LIFD. The angle between entrance and outlet channels of the cell was 120°, without any change of internal diameters or right-angles along the channels. These measures insured the elimination of bubble accumulation or formation inside the cell, and additional band broadening effect. Riboflavin standard was used as test sample to evaluate the performance of the HPLC-LIFD. The analyses of real samples containing riboflavin were demonstrated.