期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

滑膜肉瘤融合基因SYT-SSX cDNA的克隆与序列分析 被引量：1

Cloning and Sequencing of Fusion Gene SYT-SSX cDNA of Synovial Sarcomas
下载PDF
导出
摘要 利用逆转录聚合酶链式反应(RT-PCR)方法获得SYT-SSX的cDNA。克隆入PUCm-T载体后,在美国ABI DNA自动测序仪上以反向引物测序法进行核苷酸序列的测定,扩增出98 bp的特异性片段,并筛选出阳性重组克隆质粒PUCm-SYT-SSX。获得的滑膜肉瘤融合基因SYT-SSX的cDNA,为深入揭示滑膜肉瘤分子发生机理奠定基础,同时可进一步完成原核表达。 Isolate and clone SYT-SSX cDNA into PUCm-T vector using RT-PCR and then sequence it on American automatic capillary electrophoresis genetic analyzer. We amplified a 98 bp PCR product and the positive recombinant plasmid PUCm-SYT-SSX was consistent with the reported sequence. We have cloned SYT-SSX cDNA and it is ready for our further studies.
作者 李新霞 李锋 付欣鸽 陈云昭 蒋金芳
机构地区 石河子大学医学院肿瘤研究中心
出处 《石河子大学学报（自然科学版）》 CAS 2003年第4期285-288,共4页 Journal of Shihezi University(Natural Science)
关键词 滑膜肉瘤 融合基因SFF-SSX 逆转录聚合酶链式反应 测序 基因克隆 序列分析 synovial sarcoma fusion gene SYT-SSX RT-PCR sequence clone
分类号 R738.6 [医药卫生—肿瘤] Q785 [生物学—分子生物学]
  • 相关文献

参考文献21

  • 1Mackenzie D H. Synovial sarcoma: a review of 58 cases[J].Cancer, 1966,19:169.
  • 2Aubry M C, Bridge J A, Wickert R, et al. Primary monophasic synovial arcoma of the pleura: five cases confirmed by the presence of SYT-SSX fusion transcript [J]. Am J Surg Pathol, 2001,25: 776-781.
  • 3Hiroshi I, Masako I, Yuko O, et al. Synovial sarcoma of the prostate with t(x;18)(p11.2;q11.2)[J].Am J Surg Pathol, 1999,23(2) :220-226.
  • 4Pedram A,Paulo A F,Jonathan I E, et al.Primary renal synovial sarcoma[J]. Am J Surg Pathol,2000,24(8): 1087-1096.
  • 5Limon J, Dal Cin P, Sandbeng A A. Translocations involving the X chromosome in solid tumors: presentation of two sarcomas with t(X; 18)(q13;p11)[J]. Cancer Genet Cytogenet, 1986,23(1) :87-91.
  • 6Turc-Carel C,Dal Cin P,Limon J, et al. Involvment of chromosome Xin primary cytogenetic change in human neoplasia: nonrandom translocation in synovial sarcoma[J].Proc Natl Acad Sei USA,1987,84:1981-1985.
  • 7Sreekantaiah C, Ladanyi M, Rodriguez E, et al. Chromosomal aberrations in soft tissue tumors. Relevance to diagnosis, classification, and molecular mechanisms [J]. Am J Pathol, 1994, 144: 1121 -1134.
  • 8Lee W, Hank, Harris CP, et al. Use of FISH to detect chromosomal translocations and deletions: analysis of chromosome rearrangement in synovial sarcoma cells from paraffin embedded specimens[J]. Am J Pathol, 1993,143(1): 15-19.
  • 9Yang P, Hirtose S, Hasegawa T, et al. Dual colour fluorescence in situ hybridization analysis of synovial sarcoma[J]. J Pathol,1998,184(1) :7-13.
  • 10Limon J, Mrozek K, Mandahi N, et al. Cytogenetic of synovial sarcoma:presentation of ten new cases and review of the literature[J]. Genet Chromosomes Cancer, 1991,3(5) :338-345.

同被引文献7

引证文献1

石河子大学学报（自然科学版）
2003年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部