阿魏酸对活化内皮细胞粘附分子表达的抑制作用

Inhibitive effects of ferulic acid on adhesion molecules expression by activated endothelial cells

目的 观察阿魏酸对活化的人脐静脉内皮细胞表达粘附分子的影响 ,以探讨其抗动脉粥样硬化的部分分子机制。方法 用脂多糖 10mg·L- 1刺激培养的人脐静脉内皮细胞 4h诱导其表达E 选择素、刺激 18h诱导其表达血管细胞粘附分子 1;用过氧化氢 3 0 0 μmol·L- 1刺激 2h诱导其表达P 选择素 ,部分细胞在刺激前 3 0min用阿魏酸 0 2 1、0 41或 0 62mmol·L- 1预处理 ,用流式细胞术检测内皮细胞粘附分子表达水平。结果 阿魏酸抑制内皮细胞E 选择素及P 选择素表达 ,轻度抑制血管细胞粘附分子 1表达 ,同时抑制内皮细胞vWF的释放。

AIM To investigate the effects of ferulic aci d (FA) on the expression of adhesion molecules by activated human umbilical vein e ndothelial cells (HUVEC) and to elucidate one of the molecular mechanisms of FA 's anti-atherosclerosis effect. METHODS Cultured HUVEC were tr e ated with ferulic acid (0 41 mmol·L -1 or 0 62 mmol·L -1 ) and stim ulated with lipopolysaccharide (LPS 10 mg·L -1 ) 30 min later. Expression of E-selectin and vascular cell adhesion molecule-1 (VCAM-1) were de- tected b y flow cytometry (FCM) after 4 h and 18 h respectively . Cultured HUVEC were treated with ferulic acid (0 21, 0 41 mmol·L -1 or 0 62 mmol·L -1 respectively) and stimulated with H 2O 2 (300 μmol·L -1 ) 30 min later. After 2 h, expression of P-selectin on HUVEC surface wa s dedected by FCM. RESULTS Ferulic acid inhibited the expression of E-selectin and P-selectin on activated HUVEC. The inhibitive effect of feru lic acid on expression of VCAM-1 was slight. It also suppressed the secrection of vWF on activated HUVEC. CONCLUTION The inhibitive effects of f erulic acid on the expression of adhesion molecules on activated HUVEC may contr ibute to its anti-atherosclerosis effects.