他巴唑对甲状腺细胞活性氧的影响

Effect of methimazole on reactive oxygen species in porcine thyrocytes and its structure-effect relationship

目的 观察他巴唑对甲状腺细胞活性氧 (ROS)的影响 ,探讨其作用的构效关系。方法 以 2′ ,7′二氯氢化荧光素和氢乙啡啶为细胞内H2 O2 与O- ·2 的荧光探针 ,以流式细胞术观察他巴唑及其结构类似物对甲状腺细胞活性氧的影响。结果 他巴唑对甲状腺细胞内外源性ROS均有清除作用 ,且这一作用呈剂量依赖性 ;无巯基的他巴唑结构类似物咪唑对ROS无清除作用 ,而谷胱甘肽过氧化物酶抑制物二乙马来酸可部分抑制他巴唑这种作用。结论 他巴唑对甲状腺细胞活性氧具清除效应 。

AIM To investigate the scavenging effect of methimazo le treatment on reactive species(ROS) level in porcine thyrocytes and to determine its structure-effect relationship. METHODS 2',7'-dichorofluoresin diacetate (DCFH-DA) and hydroethine(HE) were adopted as fluroscent probe of intercellular hydrogen peroxide(H 2O 2)and superoxide anions(O -· 2),respectively. Their fluroscent intensity were determined by flow cytometry, and the effect of methimazole and its analogs on ROS level in thyrocytes were studied. RESULTS In thyrocytes, methimazole scavenged endogenous ROS in a dose-dependent manner, and extrinic ROS as well. But imidazole, its analogs, showsed no scavenging effect. DEM, a compound that can react with GSH irreversibly, partly reversed the effect of methimazole. CNCLUSION Methimazole can scavenge ROS in thyrocytes, and its effect may in part owe to its thiol radical which can change the ratio of GSH/GSSG.