丹参对实验性肺纤维化小鼠病理变化和核因子-κB表达的影响

Effect of salvia miltiorrhiza on the change of pulmanory pathology and expression of nuclear factor-kappa B in mice pulmonary fibrosis

目的 探讨丹参水煎液对实验性肺纤维化形成的影响。方法 通过气管内注射博莱霉素复制小鼠肺纤维化模型 ,给予丹参水煎液治疗后 ,以肺指数、肺组织病理学变化及形态学定量测量 ,并用免疫组织化学方法测定肺组织中NF κB的表达 ,以观察丹参水煎液对肺纤维化形成的影响。结果 丹参水煎液能抑制实验性肺纤维化小鼠的肺指数及肺组织中NF κB的异常升高 ,减轻其肺部的病理损害。

AIM To study effect of Salvia miltiorrhiza on the change of pulmanory pathology and expression of nuclear factor-kappa B in mice pulmonary fibrosis. METHODS Mice models were reproduced by injecting bleomycin to the trachea. Then the mice were treated with Salvia multiorrhiza. Observed Salvia miltiorrhiza effect on the mice models included pulmonary index, pulmonary pat hology and expression of NF-κB in pulmonary tissue by immunohistochemical technique. RESULTS Salvia miltiorrhiza depressed pulmonary index, reduced pulmonary pathological damage. At the same time, we also observed that it suppressed the abnormaly increased expression of NF-κB in pulmonary tissue of pulmonary fibrosis mice. CONCLUSION Salvia miltiorrhiza has good effect on reliefing pulmonary fibrosis reproduced by bleomycin.