摘要
Objective:To directionally clone the ompl gene from Chlamydia trachomatis(Ct)F Genotype onto a plasmid vector for constructing a rudimentary DNA vaccine.Methods:The complete ompl gene from genomic DNA of Ct F genotype wild species was amplified with primers designed by computer.The recombinant gene was obtained by restriction enzyme cutting,linking the gene with the plasmid vector in vitro,transforming the recombinant gene into bacteria,and extracting the DNA from the bacteria.Results:DNA extracting from the bacteria was composed of the impl gene and plasmid,which is identified by three methods of singular restrictive enzyme cutting,double restrictive enzyme cutting and PCR.Conclusion:Cloning of the ompl gene from the Ct F genotype means that a rudimentary DNA vaccine was successfully constructed.
Objective: To directionally clone the ompl gene fromChlamydia trachomatis (Ct) F genotype onto a plasmid vectorfor constructing a rudimentary DNA vaccine. Methods: The complete ompl gene from genomic DNA of CtF genotype wild species was amplified with primers designedby computer. The recombinant gene was obtained byrestriction enzyme cutting, linking the gene with the plasmidvector in vitro, transforming the recombinant gene intobacteria, and extracting the DNA from the bacteria. Results: DNA extracted from the bacteria was composed ofthe ompl gene and plasmid, which is identified by threemethods of singular restrictive enzyme cutting, doublerestrictive enzyme cutting and PCR. Conclusion: Cloning of the ompl gene from the Ct Fgenotype means that a rudimentary DNA vaccine wassuccessfully constructed.
