吸附树脂提取的大豆根分泌物种类的GC-MS分析

GC-MS ANALYSIS ON THE KINDS OF SOYBEAN ROOT EXUDATES EXTRACTED WITH ADSORPTION RESIN

采用GC -MS分析法 ,鉴定了XAD - 4吸附树脂连续提取所获得的大豆根分泌物的种类 ,并研究了化感物质 (邻苯二甲酸 )对大豆幼苗生长的化感作用。结果表明 :根分泌物连续捕获系统(XAD - 4吸附树脂 )获得的根分泌物种类丰富 ,主要是长链脂肪酸、苯甲酸及其衍生物 ,直链醇、烯醇、酚、酮、苯胺、酯和一些烃类及其衍生物等有机化合物 ,其中很多都被报道是化感物质。在一定浓度范围内 ,邻苯二甲酸对大豆幼苗生长具有显著的化感抑制作用。表明大豆根分泌物中存在抑制大豆幼苗生长的化感物质 ,由此可引发大豆连作障碍。

Soybean root exudates extracted with XAD-4 resin were identified by GC-MS method, and the allelopathy of chemical compounds (1,2-benzenedicarboxylic acid) on soybean seedling were inresti gated in this study. The results showed that root exudates extracted with XAD-4 resin was identified as long-chain fatty acids, benzoic acids and its derivates, straight-chain alcohol, enol, phenol, ketone, ester and some hydrocarbon, most of which were also allelopathic compounds reported. In certain range of concentrations, the 1,2-benzenedicarboxylic acid significantly inhibited the growth of soybean seedlings. The results showed that soybean root exudates contained allelopathic compounds that inhibited seed germination and seedlings growth, and this might cause soybean continuous cropping barriers.