雌激素受体在肺腺癌和鳞状细胞癌中的表达研究

The experssion of estrogen receptor in human adenocarcinoma and squamous cell carcinoma of lung

目的 研究雌激素受体 (ER)α和 β在良性肺组织和肺癌组织中的表达及其与临床和病理学特征的关系。 方法 采用免疫组织化学方法检测ERα和 β在 18例肺腺癌和 2 9例肺鳞状细胞癌 (共有 35例含癌旁肺组织 )及 12例良性肺病变组织中的表达。切片内阳性细胞数≥ 2 0 %为ER阳性。结果 ERβ在肺癌组织中的表达主要定位于细胞核 ,其在肺癌组织中的表达率为 4 4 .7% (2 1/ 4 7) ,而在癌旁肺组织和肺良性病变组织中无明显表达。ERβ在鳞状细胞癌、腺癌中阳性表达率分别为2 7.6 %(8/ 2 9)、72 .2 % (13/ 18) ,腺癌组织中ERβ表达率显著高于鳞癌 (P <0 .0 5 )。ERα在肺癌、癌旁肺组织和肺良性病变组织中均未见表达。

Objective To investigate the experssion of extrogen receptor α and β (ERα and β) in human adenocarcinoma and squamous cell carcinoma of lung and in benign lung tissues as well as the correlation between ER expression in lung cancer and clinicopathiological characteristics.Methods The expression of both ERα and β in 47 specimens from patients with adenocarcinoma or squamous cell carcinma of lung and in 12 specimens from patients with benign lung diseases was investigated by immunohistochemistry. It was scored as positive, when ≥20% of total cells in an area with a homologous structure distinctively stained. Results The expression of ERβ was localized mainly in nuclei of lung cancer cells, whereas no clearly staining was found in benign lung tissues. The positive percentage of ERβ expression in human lung cancer was 44.7%(21/47). The expression of ERβ was statistically higher in adenocarcinoma, 72.2%(13/18) than that in squamous cell carcinoma, 27.6%(8/29)(P< 0.05). ERα was not detectable in lung cancer or in benign lung tissues.Conclusion ERβ was overexpressed in both human adenocarnoma and aquamous cell carcinoma of lung, which suggests that ERβ may play a role in the development of lung cancer, in particular in adenocarcinoma of lung.