结缔组织生长因子在瘢痕疙瘩中表达的研究

A Preliminary Study on the Expression of Connective Tissue Growth Factor in Keloids

目的探讨结缔组织生长因子(CTGF)在瘢痕疙瘩发病中的作用。方法应用半定量逆转录聚合酶链反应技术检测30例瘢痕疙瘩患者皮损及对应邻近未受累皮肤中CTGFmRNA的表达,并以15例正常人皮肤组织作为对照。同时应用SP免疫组化技术对5例瘢痕疙瘩组织和5例正常人皮肤标本进行了检测。结果CTGFmRNA在瘢痕疙瘩及其边缘正常皮肤中的表达均明显高于正常人对照,差异有显著性(P<0.01)。瘢痕疙瘩CTGF的表达高低与病程无相关性(P>0.05)。免疫组化研究证实CTGF在瘢痕疙瘩中呈强表达,而在正常人皮肤中无表达。在瘢痕疙瘩组织边缘,CTGF表达呈现由强至弱的过渡现象。结论CTGF在瘢痕疙瘩中持续高度表达,提示其与瘢痕疙瘩的慢性纤维化有关。CTGF可能成为临床治疗瘢痕疙瘩的一个有力靶位。

Objective To investigate the role of connective tissue growth factor(CTGF)in the pathogenesis of keloid.Methods The expression of CTGF mRNA was detected with semiquantitative RT-PCR technique in keloid tissue and adjacent uninvolved skin from30patients.The expression of CTGF pro-tein was determined by SP immunohistochemistry in5keloid tissues and5normal skin samples.Results Compared with normal controls,the mean levels of CTGF mRNA expression were significantly increased in both keloid lesions and adjacent uninvolved skin(P<0.01).There was no correlation between CTGF mR-NA expression and the course of keloids(P>0.05).In immunohistochemistry study,CTGF protein was strongly expressed in keloid tissue,whereas it was not expressed in normal skin.Transitional phenomenon was observed from strong to weak expression of CTGF protein at the edge of keloid tissue.Conclusion These findings suggest that CTGF may be related to the development of persistent fibrotic tissue formation in keloids.CTGF may be a new potential target for the therapeutic intervention of keloids.