FK506对雪旺细胞体外增殖能力的影响

Acceleration of Schwann Cells Proliferation in Vitro Using FK506

目的 研究普乐可夫(FK506)促进周围神经再生的作用和机制。方法 将纯化的兔雪旺细胞分3组:10μg/L FK506、100μg/L FK506和空白对照组,继续培养10 d,相差显微镜观察计数,绘制各自的增殖曲线。在24和72 h对各组细胞进行流式细胞分析。结果 FK506组雪旺细胞生长良好,而成纤维细胞可以发现细胞肿胀,胞质内出现空泡;活细胞计数对照组雪旺细胞的倍增时间为8d,10μg/L组和100μg/L组倍增时间6.2d。用FK506培养24、72 h后对雪旺细胞增殖的流式细胞分析处于S期的雪旺细胞较对照明显增高(P< 0.01)。结论 FK506有促进体外培养的雪旺细胞快速增殖和抑制成纤维细胞生长的作用。免疫抑制剂FK506的双重作用在神经再生治疗中具广阔的应用前景。

Purpose: To explore the role and mechanism of FK506 in promoting peripheral nerve regeneration. Methods: The purified Schwann cells from rabbits were divided into three group: 10 μg/L FK506 and the control group. Following 10 days in vitro, the cells from three groups were counted with phase contrast microscope every day to draw proliferation curve respectively. Flow cytometries were carried out in each group after 24 and 72 h respectively. Results: The Schwann cells grew well and a lot of balloons in the shruken fibroblasts cytoplasm were discovered in FK506 groups. The doubling time of control group was 8 days, the 10 and 100 μg/L FK506 group were 6.2 days respectively. The flow cytometry of the three groups showed that Schwann cells in S phase of the two FK506 groups were significanly higher than the control group(P < 0.01). Conclusions: FK506 could promote Schwann cell proliferation and restain the growth of fibroblast in vitro. The double role of immunosuppressive agent FK506 may have broad application prospect in treatment of nerve regeneration.