鹌鹑口服氯化镉后,镉在主要器官中的分布及临床变化

CADMIUM DISTRIBUTION IN MAJOR TISSUES AND CLINICAL SINGS FOLLOWING CADMIUM CHLORIDE ADMINISTRATION ORALLY TO QUAILS

54只26日龄雄性鹌鹑等分为9组,分别喂给含相当于采食量的0……100mg/kg镉的氯化镉小面丸,上、下午各一次。连续28天。发现除高剂量组(75、100rag/kg)部分动物死亡外。各饲镉组动物均有采食量减少,体增重减慢、消瘦、贫血、睾丸缩小、骨质疏松等临床症状。镉主要蓄积在肝、肾中。各器官内镉积累量与饲料中供镉量间呈明显的正相关(r>0.93),在肝、肾细胞浆液中,90％以上的镉与金属硫蛋白(MT)结合,仅少量镉与大分子量蛋白质(HP)结合。

Fifty four 26-day-old malc quails wcre allocated into nine groups of six. Birds received 0, 0, 1, 5, 10, 25, 50, 75 and 100 mg/kg Cd (as CdCl_2) correspond to the ration orally for 4 wceks. 5 of 6 birds in 100mg/ kg-group dicd during the fifth to 16th, and 3 of 6 birds in 75 mg/ kg-group dicd during the sixth to 12th day. The clinical signs include: depression, decreased food intake and growth rate, emaciation, anemia, convulsion, poor bonc mincralization and rcduced wcight, reduced testicle size and weight, and kidney swelling. Although Cadmium lethal does was as high as 75mg/kg, lmg/kg level, reduced testicle size and 5rag/kg level decreased hemoglobin concentration. Cd accumulated mainly in liver and kidney. Cd contents in all tissues measured increased progressively with increase in Cd dose, At the 1 to 25 rag/kg-levcl Cd concentrations in kidney were higher.than those in liver, however, at the 50 to 100 mg/kg-lcvel Cd concentrations in liver were higher than those in kidney. It suggests that Cd deposition is dose-dependented. Hepatic copper concentrations decreased and kidney copper concentrations increased progressively with increase in Cd dose. The distribution of Cd in fractions of liver and kidncy cytosols showed 2 peaks, which were Cd bound to high molecular weight proteins and to metallothionein respectively. It also revealed that a large proportion of the cytosolic Cd was associated with ligands of mctallothionein.