陆地棉品种抗枯萎病有关生理生化性状的研究

A Study on Some Physiological and Biochemical Characters Regarding Fusarium Wilt Resistance in Upland Cotton

以对枯萎病抗性不同的陆地棉品种为材料进行有关生理、生化等性状研究。结果表明:对枯萎病抗性不同的陆地棉品种间,棉苗下胚轴横切面解剖结构、苗期和蕾期叶片可溶性糖及可溶性蛋白质含量、棉花枯萎病菌毒素提取液对种子发芽和幼苗的毒害作用、兔血凝集反应等方面均存在显著差异;植株感病后,过氧化物酶、超氧化物歧化酶、酯酶、多酚氧化酶4种酶的同工酶也呈现明显差异,从而可将上述性状做为预先检测棉花品种抗枯萎病指标的重要依据。

Upland cotton varieties resistant and susceptible to Fusarium wilt were tested to establish some new resistance indices. The results indicate that there are significant differences between resistant and susceptible varieties in the cross section structure of hypocotyl, the contents of soluble sugars and proteins in leaves at the seedling and squaring stages,the response of seeds and seedings to the toxin extracted from Fusarium oxysporum,and the coagulability of cotton seed extract with red blood cells of rabbits. The isozyme zymograms of per-oxidase,esterase ,polyphenoloxidase and SOD are also obviously different between the resistant and susceptible varieties. These differences could be used as indices to early predict the resistance of cotton to Fusarium wilt. There are also differences in contents of phenol and 17 kinds of amino acids in seeds between the two kinds of varieties,but they are not significant.