培养基组分对四倍体水稻试管速繁中不定芽丛增殖的影响

Effect of Medium Compohents on Multiplication of Adventitious Bud Clusters in Rapid Test-tube Propagation of Autotetraploid Rice Clones

以 MS 培养基为基础,研究了诱导四倍体水稻体细胞试管苗形成及其繁殖的几个因素。(1)诱导四倍体水稻试管苗(芽)大量形成不定芽的必要条件是 KT≥11.57mg/l(KT/NAA≥1000),获得最大不定芽丛生速率的 KT 水平为23.20mg/l(KT/NAA=2000)。(2)氮、磷水平对不定芽的诱导及增殖速率均有明显的影响,它们的最佳水平分别为10100mg/l KNO_3+1320mg/l(NH_4)_2SO_4(纯总氮量120mmol/l。NO_3^--N/NH_4^+-N=5)和1190mg/l KH_2 PO_4。(3)继代培养第十天添加 CLNⅡ补充营养液(23.20mg/l KT.0.01mg/l NAA,MS 有机附加物及铁盐.800mg/l水解乳)能够显著地提高不定芽的丛生速率。(4)蔗糖利用率和激素水平密切相关,其在23.20mg/l KT+0.01mg/l NAA 条件下达到最大值(85.5%)。

The main results are summarized as follows:(1) KT 11.57mg/1 (KT/NAA 1000) is essential for the induction of adventitious buds on the tube-plantlets of autotetraploid rice clones.Maximum induction and propagation of adventitious buds occurred in response to KT- 23.20mg/1 (KT/NAA=2000) level.(2) Different nitrogen and phosphorus levels have strong influence on the propagation of tube-plantlets.The best combination is 10100mg/1 KNO_3+ 1320mg/1 (NH_4)_2SO_4(total net N is 120mmol/1 and NO_3^--N/NH_4^+-N ratio is 5) and 1190mg/1 KH_2PO_4.(3) The additional nutrient solution CLN Ⅱ (consisting of 23.20mg/1 KT,0.01mg/1 NAA,800mg/1 LH.organic supplements and Fe salts of MS)could induce a dramatical number of plantlets when added into culture vessels on the tenth day after transferring.(4) The uptake of sucrose by tube-plantlets was related with KT level.The consuming-rate of sucrose reaches the maximum value (85.5%) under condition of 23.20mg/1 KT+ 0.01mg/1 NAA in our test.