稻瘟病菌孢子保存技术

Preservation Technique of Pyricularia oryzae Conidia

将载有稻瘟病菌孢子的滤纸置于不同温度和干燥条件下保存,经分期测定,室内干燥保存,经10个月,不论是孢子活力和致病性一般均可维持原有水平。低温干燥保存则能保存更长的时间。这样解决了目前人工接种中稻苗和孢子的培养难以配合的问题。

Freshly produced conidia were collected from barley grain medium by washing with distilled water,the spore suspension is filtered onto filter paper in a funnal,dried at 30-35℃, and stored in desiccator at indoor,4℃ and -20℃.The conidia retained their original level of viability and pathogenicity after 10 months indoor,there are further preservation times at 4℃ and -20℃.Thus,the problem is solved that culture rice seedling can not synchronize with sporulation culture of blast fungus during the artifical inoculation.