两株人胰腺癌裸小鼠胰腺内原位移植模型的建立及其生物学特性

ORTHOTOPIC TRANSPLANT MODEL OF HUMAN PANCREATIC CANCER IN NUDE MICE AND THEIR BIOLOGICAL PROPERTIES

报告国内首次建立两株人胰腺癌裸小鼠胰腺内原位移植模型。方法系将人胰腺癌手术标本直接移植于BALB/C-nu/nu裸小鼠胰腺内,肿瘤异体移植和连续传代获得成功。研究了其生物学特性。结果表明:PTNMP-1已传至9代,PTNMP-2已传至6代。肿瘤移植成功率为95％～100％。两株移植瘤均能产生大量的癌胚抗原CEA,染色体分析证实为人类肿瘤细胞核型。在荷瘤裸小鼠体内出现淋巴道、血道转移,光镜、电镜检查结果证明原位移植瘤的形态学与原手术标本完全一致。此模型可用于胰腺癌的侵袭、转移及实验治疗。

Two cell strains from human pancreatic cancer taken surgically were transplanted to the pancreas of pureline BALB/C-nu/nu nude mice and 9 generations of PTNMP-1 and 6 generations of PTNMP-2 were obtained. Biological properties were studied. It yielded a transplant success rate of 95% - 100% and large amount of CEA. Analysis of the karyotype confermed that they retained the karyotype of human cancer cells. Some lymphatic and blood stream metastasis and invasion could be found in the nude mice orthotopically transplanted with tumour, showing that the transplanted tumour had characteristics identical with the donor tumour.