选择性肠道清洁疗法防治重症烫伤大鼠肠道细菌易位的研究

PREVENTION OF BACTERIAL TRANSLOCATION BY SELECTIVE DECONTAMINA TION OF THE DIGESTIVE TRACT IN THERMAL-INJURED RATS

本实验采用人鼠40％Ⅲ°烫伤模型,动态观察了选择性肠道清洁疗法(SDD)对肠道细菌易位的影响。结果证实,治疗组动物肠粘膜菌群中G肠杆菌及酵母菌完全被抑制,烫伤后1、3、5d细菌易位率都显著下降(P<0.05～0.01),且动物预后明显改善。伤后5d存活率显著高于对照组(P<0.05)。该研究结果提示,预防性应用SDD对于重症烧伤后肠道细菌易位具行一定的防护作用,从而可能有助于阻止肠源性感染的发生与发展过程。

The present investigation was performed to study the effect of selective decontamination of the digestive tract (SDD) on bacterial translocation from the intestine in rats after thermal injury (40% full-thickness scald injury). In the SDD-treated group, Enterobacteriaceae and yeasts were eradicated from the gut, while the mucosal flora consisting of mainly anerobes, was well preserved, within 3 days, and the incidence of bacterial translocation to the mesenteric lymph nodes (ML.N) and visceriae was significantly lower on postburn day 1, 3 and 5 (P<0.05- 0.01). The five-day survival rate of the group treated with SDD was remarkedly higher than that of the control group (x2 = 4.57, P<0.05). These data indicated that selective decontamination was highly effective in preventing bacterial translocation from the gut, and it might be helpful in abating gut origin septic state following severe burn injury.