荧光定量PCR检测女性商业性性工作者性传播感染

Detection of sexually transmitted infections among detained female commercial sex workers by fluorescent quantitative PCR assay in Shenzhen.

目的 : 了解女性商业性性工作者 (FSWs)沙眼衣原体 (Ct)、淋球菌 (NG)、单纯疱疹病毒 (HSV)及人乳头瘤病毒 (HPV)等常见性传播感染 (STI)的患病情况。方法 : 选取深圳市收容教育所 2 0 0 2年 11～12月因卖淫而收容教育的 130例FSWs作为研究对象 ,采用荧光定量PCR(FQ -PCR)检测宫颈分泌物Ct、NG、HSV及HPV感染。结果 : FQ -PCR检测 130例FSWs宫颈分泌物的Ct阳性检出率为 32 .3%、NG阳性检出率为 16 .2 %、HSV为 5 .4 %、HPV6 11、HPV16和HPV18分别为 7.7%、2 3.8%和 12 .3%。STI总的阳性检出率为 6 0 .8% ,混合感染率为 2 5 .4 %。结论 : 女性商业性性工作者的STI患病率高 。

Objective:To determine the prevalence of sexually transmitted infections (STI) among female commercial sex workers (FCSWs) in Shenzhen. Methods:Fluorescent quantitative PCR was used to detect major organisms of sexually transmitted infections among 130 detained FCSWs in a women's reeducation center in Shenzhen.Results:The prevalence rate of CT,NG,HSV,HPV6/11,HPV16 and HPV18 for cervix secretion is 32.3%,16.2%,5.4%,7.7%,23.8% and 12.3% respectively. The overall positivity rate of STIs was 60.8% (79/130), and the prevalence rate of mixed STIs was 25.4%. Conclusion:The prevalence of STI was high among FCSWs in Shenzhen.