大肠癌超微结构、ras癌基因及粘液变化研究

STUDY ON ULTRASTRUCTURE, RAS ONCOGENES AND CHANGES IN MUCUS OF COLON CARCINOMA

用多种较先进技术对大肠癌进行研究。结果证明,(1)电镜下所观察到的癌细胞微绒毛粗大、密集、小根深埋在胞质顶部是大肠管状腺癌的超微结构标志。人体内癌细胞侵袭方式的微细形态变化与体外器官培养中所见相似。(2)ras癌基因P21检测,159例大肠癌中104例(65.4％)过量表达,提示大肠癌与ras癌基因活化有密切关系。5种单抗测试的122例中阳性率在83.4％～95.7％之间,说明大多数含有大肠癌肿瘤相关抗原。对提高诊断与治疗水平有重要作用。62例粘液腺癌和30例印戒细胞癌,硫酸粘液显著减少,唾酸粘液明显增多。后者为含羧基酸粘液的糖蛋白,有利于癌细胞脱离群体,向周围侵袭及扩散。(3)134例核仁组成区相关嗜银蛋白(AgNoR)定量检测,每个核颗粒数为13.05±1.48((?)±s)。未分化癌AgNOR量多(19.24),而乳头状腺癌、管状腺癌、粘液腺癌波动在10.56～11.83之间,提示AgNOR定量与大肠癌恶性程度有关。

The characteristics of ductal adenocarcinoma of colon under eieciror: microscopy were stout and dense microvill, with small roots buried in the top of cytoplasm. The ullrarnorphological changes of carcinomatous infiltration was similar to the picture observed in vitro cultivation of cancer cells.In 104 cases out of 159, there was an over expression of ras oncogenes P21, indicating that carcinoma of colon was related to activation of ras oncogenes. Examination with monoclonal antibodies yielded a positive rate of 83.4%- 95.7%, indicating that most of the carcinomas had colon related antigens. This phenomenon should play an important role in raising the diagnostic accuracy, and it also should serve as an indicator of effective treatment. In 62 cases of mucoid adenocarcinoma and 30 cases of signet ring cell carcinoma, there was a marked reduction of mueoitin sulfate with an increase in sialic acid mucus. The letter was a glycoprotein containing hydroxyl mucus, and it facilitated the separation of cancer cells to infiltrate and metastasize.in 134 cases, AgNOR was quantified, and there were 13.05± 1.48 granules in each mucleas. There was more abundant AgNOR in undifferentiated cancer, while the number of AgNOR fluctuated between 10.56 to 11.83 in papillary adenocarcinoma, ductal adenocarcinoma, and mucoid adenocarcinoma. indicating the qunantity of AgNOR was related to degree of malignancy in colonic carcinoma.