休克大鼠微循环中白细胞流动分布的变化及虎杖4号的治疗作用

THE CHANGE IN FLOW DISTRIBUTION AND THE EFFECT OF POLYGONUM CUSPID ATUM ON RAT MICROVASCULATURE DURING HEMORRHAGIC SHOCK

应用微循环图像多参数计算机分析系统,测量了大鼠失血性休克过程中骨骼肌微循环中白细胞流动分布的变化,发现第三级细静脉内中央轴流白细胞的流速减慢,移边并开始沿血管内皮滚动,最后粘着于血管内皮。输血时,血流加快,粘着白细胞开始沿管壁滚动,并加速进入中央血流。计算了反映白细胞和内皮细胞作用程度的指标:总体白细胞-内皮细胞接触时间(TLECT),发现休克时TLECT明显延长。查明在失血性休克晚期,要解除白细胞和内皮细胞的相互作用,单纯提高血压和壁切应力是不够的,尚需进一步探索降低白细胞和内皮细胞粘附力的方法。虎杖4号可能同时具有提高壁切变率和降低粘附力的效应。

The flow distribution of leukocytes in the microvasculature of rat skeletal muscle during hemorrhagic shock was measured by Microcirculation Image Multiple Parameters Computer Analysis System (MIMPCAS). It was found that the change of leukocyte flow distribution had taken place in V3 during hemorrhagic shock as follows: central axial-flowing leukocytes moved slowly, marginated to the vessel wall then started to roll along the wall, and stuck to the endothelium at last. During transfusion blood flow became faster, sticking leukocytes were rushed out to be rolling along the vessel wall and accelerated to enter the central stream. Total leukocyte -endothelium contact time (TLECT), which is a quantitative index for leukocyte endothelium interaction, is calculated, and it was found that TLECT was prolonged in shock state. The results show that it is not enough to increase blood pressure and shear stress to liberate sticking leukocytes, and it is necessary to search for a new approach to decrease the adhesive force between leukocyte and endothelium. Polygonum cuspidatum may not only increase wall shear rate but also decrease the adhesive force.