先天性巨结肠RET蛋白免疫组化研究

Immunohistochemical Study of RET Protein In Hirschsprung's Desease

目的 了解RET蛋白与先天性巨结肠(HD)组织改变之间的关系,以探讨HD的发病机理.方法 利用抗RET蛋白的三种抗体,以及抗磷酸酪氨酸抗体采用免疫组织化学方法,对42例散发性HD肠组织中RET蛋白表达情况进行研究,10例正常结肠作对照。结果 抗RET蛋白的三种抗体在HD扩张段、移行段、对照组均显强阳性染色,P>O.05;在狭窄段大多数表现阴性染色,少数可见强阳性或弱阳性染色,与前三者比较P<O.05。抗磷酸酪氨酸抗体在HD扩张段、对照组显示出强阳性染色,P>O.05;HD移行段与狭窄段大多数呈现阴性或弱阳性染色,P>O.05。前两者与后两者比较P<O.05。结论RET蛋白对HD的形成有重要的作用。

Objective To understand the relationship between RET protein and the intestinal tissue change of congenital megacolon (Hirschsprung's Disease, HD), in order to explore the molecular and cellular pathogenesis of the HD. Methods Expression of RET protein in the in testinal tissue from 42 cases of sporadic HD patients, (HD group), and 10 cases of colon tissue without HD (control group), were detected by using immunohistochemical technigue with three RET protein antibodies and antiphosphotyrosine antibody, respectively. Results A strongly positive staining was seen in the ganglion fibres of the dilated intestine segment of the HD (HD-D), transitional intestine segment of the HD (HD-T) and control colon tissue (control group), p>0.05. The negative or a lightly positive staining had shown where in the nerve trunks of stenotic segment of HD, as compared with HD-D, HD-I and control group, it was different from the formers p<0.05. In the diated intestine segment of HD and control group had shown strongly positive staining with antiphosphotyrosine antibody p>0.05; but the transitional intestine ganglion cells of HD and stenotic segment of HD was negative staining or a lightly positive staining p>0.05. Both the diated segment of HD and control group as compared with the trasitional and stenotic segment, there was remarkable difference p<0.05. Conclusion RET protein can play an importent role in the pathogenesis of HD.