冷损伤大鼠血浆中纤维结合蛋白含量改变

CHANGES OF FIBRONECTIN CONTENT IN THE PLASMA OF COLD INJURED RATS

本文用单向免疫扩散法观察了不同程度冷损伤大鼠血浆中纤维结合蛋白(FN)含量的改变.204只体重为250±20g Wistar大鼠麻醉后双后足浸在-25℃乙醇中使组织温度分别达到-20℃、-10℃和0℃,在冻后4h、24h、3、5和7天测定FN含量.结果表明冷损伤后血浆中FN含量增加,其增加程度与冷损伤程度密切相关.这可能是由于组织冷冻而引起的血管内皮细胞损伤所致,它在血液循环障碍和代谢障碍病理过程中具有重要作用.

This paper reports the changes of fibronectin (FN) content by unidirectional immunodiffusion method in the plasma of rats with different degree of cold injury. 204 Wistar rats weighing 250±20g were used.The hind feet of anesthetized rats were immersed in alcohol at -25℃ until the temperature of tissue reached -20℃,-10℃ or 0℃.The plasma FN contents were determined at 4,24 hours,3,5 or 7 days after cold injury.The results demonstrated that there was increase of the FN contents,which were closely related to the degrees of cold injury.It is possible that vascular endothelium was damaged by tissue cooling,which may play an important role in the pathological processes of blood circulatory and metabolic dysfunctions.