高效液相色谱法检测血清帕罗西汀药物浓度

Determination fof paroxetine concetration in high performance liquid chromatography

目的 建立一种简便、快速、灵敏的反相高效液相色谱(HPLC)紫外检测法测定血清帕罗西汀药物浓度。方法 选用C18柱,波长295 nm,丙咪嗪为内标,移动相为甲醇:水:三氯甲烷:异丙醇:四甲基乙二胺:冰乙酸=282:140:1.6:0.8:1.6:2,流速1.0 ml/min,以甲苯、正己烷(1:4)为萃取液。结果 帕罗西汀和内标的保留时间分别为3.40 min和4.20 min,批内和批间平均回收率各为98.4％-100.0％和98.8％-101.8％,RSD分别小于8.0％和5.1％,最低有效检测浓度为3 ng/ml。结论 本法操作简便、快速而精确,具有一定的实用价值。

Objectives: To develop a simple and rapid HPLC method for the determination of the blood level of Paroxetine. Method: The analysis involved a C18 column as stationary phase and methanol. distilled water, isopropanol, trichloromethane, TMEDA, HAc as mobile phase. The flow rate was 1. 0 ml/min-1, UV detection wavelength was 295 mm, Imipramine was used as internal standard. The serum samples were extracted with toluene and n-hexane. Results: Intra-day and inter-day RSD were less than 8. 0% and 5. 1% respectively the recovery rates of intra-day RSD were less than 8. 0% and 5. 1% respectively the recovery rats of intra-day and inter-day were 98. 4%-100. 0% and 98. 8%-101. 8% respectively. Quantification limits were 3 ng/ml. Conclusion: The method is simple, rapid, sensitive and accurate for clinical practice.