葡萄籽原花青素抗促癌作用的实验研究

Inhibitory Effect of Grape Seed Procyanidins on Tumor Promotion

以二甲基苯蒽(DMBA)作为启动剂，以巴豆油作为促癌剂诱 发 小鼠皮肤乳头状瘤，原花青素(PV2000)能显著性对抗巴豆油对皮肤乳头状瘤形成的促进作用 ，使小鼠生瘤百分率降低，平均每鼠生瘤个数显著减少，但对DMBA启动阶段的抑制不强。并 发现NO与小鼠皮肤乳头状瘤的形成呈正相关，原花青素对巴豆油促癌过程的抑制作用与降低 皮肤组织中NO含量有一定关系。此外，原花青素尚能显著性抑制促癌物巴豆油所致的小鼠耳 廓肿胀。葡萄籽原花青素小鼠ip给药的LD50为144.7 mg/kg，ig 给药的LD50 为3 535 mg/kg。

To study the effect of procyanidins obtained from the seed of Vitis vinifera L. in preventing tumor initiation an d p romotion induced by dimethyl benz ［α］ anthracene (DMBA) and croton oil. Two sta ge mouse skin papilloma was initiated by DMBA and promoted by croton oil, and pr ocyanidin (PV2000) was used to assess its inhibitory effect on the initiation an d promotion of the tumor. It was found that PV2000 could obviously decrease the incidence of papilloma during the croton oil promotion stage, but without effect on the DMBA initiation stage. It was found for the first time that nitric oxi d e (NO) is possitively related to the promotion of mice papilloma, and procyanidi ns were found to be able to decrease the level of NO in the infected skin during croton oil promotion stage, as shown by its inhibitory effect on mice ear edema induced by croton oil. Acute toxicity tests on procyanidins showed that its LD 50, ig mice was 3 535 mg/kg and ip mice 144.7 mg/kg. Procyanidins from g rape seed proved to be safe and worth for further development.