膀胱癌组织mdm2,p53基因蛋白的表达及意义

Expression and significance of mdm2, p53 in carcinoma of urinary bladder

检测膀胱癌组织中mdm2基因蛋白和p53基因蛋白表达并探讨二者与肿瘤分期、分级、复发的关系。方法 采用免疫组织化学技术(ABC法)对60例膀胱移行细胞癌(TCC)组织中mdm2基因蛋白和p53基因蛋白的表达进行了检测。结果 mdm2基因蛋白表达主要见于中、高分化的肿瘤细胞,并随肿瘤临床分期的增高,表达率逐渐下降,初发者比复发者表达率高(P<0.05),而p53基因的表达则与其相反。两者异常表达呈负相关(γ=-0.4737,P<0.05),均与肿瘤生长方式、年龄、性别无关。结论 mdm2基因蛋白与p53基因蛋白的异常表达与膀胱癌的临床分期、病理学分级及肿瘤复发有密切关系。

To study the relation of the expression of mdm2 and p53 to staging, grading and relapsing ofbladder cancer. Methods With immunohistochemistry(ABC),the expression of mdm2 and p53 were detected on sections of 60 patients with transitional cellcarcinoma of urinary bladder. Results mdm2 expression was mainly found in the tumors of intermediate and high dif ferenciation, and the expression was getting weeker with the increase of clinical stagings. A higher expression of mdm2 was shown in Tri-1 tumor (p<0. 05). The expression of p53 showed a contrary pattarn. There was a negative correlation between expressions of these two genes(r= -0. 4737. p<CO. 05). Both of them were not associated with growth pattern of the tumor, the age of patients and sex. Conclusions The expressions of mdm2 and p53 were closely related with the grading, staging and recurrence of the tumor.