低剂量雄黄诱导NB_4细胞凋亡的研究

Study on the Lower Dose Realgar Induced NB_4 Cell Apoptosis

目的:探讨复方黄黛片中主要成分雄黄治疗急性早幼粒细胞白血病的作用机理。方法:应用台盘蓝排染法、流式细胞仪、DNA电泳、免疫印迹等多项方法,体外研究雄黄对NB4细胞的促凋亡作用。结果:雄黄对NB4细胞的生长有抑制作用,并有促凋亡效应,对细胞周期的影响表现为G2M期的阻滞,药物处理的早期细胞凋亡,未见伴有bc1-2的下调。结论:雄黄作用的早期主要以细胞凋亡为主,并且可能通过其他途径诱导细胞凋亡。

Objctive:To explore the mechnism of realgar in the treatment of acute promyelocytic leukemia(APL).Methods:In vitro,Reglar induced apoptosis of APL cell line NB 4 and its mechanisms were studed by flow cytometry,DNA gel electrophorcsis and western bloting analysis.Results: Realgar inhibited NB 4 cells the growth and induced its apoptosis whitout down regulation bcl-2;Flow cytometric analysis of cell cycle distribtion showed that realgar arrested NB 4 cells in G 2/M phase.Conclusion:Renalgar induced apopotsis of NB 4 cells without changing in expression of bcl-2 at early stage.