针刺对脑缺血及再灌注家兔脑水肿、自由基病理学损伤的保护作用及相关性分析

Protective Effect of Acupuncture on Schema and Repercussion Injury of Free Radical Pathobiology,Edema in Rabbit Brain and their Correlation Analysis

目的:探讨针刺对实验性脑缺血及再灌注家兔脑水肿、自由基病理学损伤的影响及相关性分析。方法:利用皮瓣包埋方法结扎椎底动脉,造成急性不完全性脑缺血及再灌注模型,给予针刺治疗观察其对实验性脑缺血再灌注家兔的脑组织含水量、自由基含量及Ca2+含量的对比性观察。结果:脑缺血及脑缺血再灌注组均存在明显的脑组织水肿及自由基含量的升高,以缺血再灌注组最为严重(P<001),Ca2+含量增加则以缺血6h组最为显著,并且缺血再灌注组的自由基水平与脑水肿具有明显的相关性(r=0.8076P<0.05)。针刺可明显改善上述指标。尤以缺血再灌注组为著。结论:针刺对实验性脑缺血再灌注家兔脑水肿、自由基病理学损伤具有保护作用。

Objective: To examine whether acupuncture tretment improve the cerebral edema and the free radical pathobiological injury induced by cerebral ischemia and reperfusion in rabbits and their correlation.Methods:Animal models with acute and incomplete cerebral schema and repercussion were made by left vertebral artery libation and skin flap investing method.Having been given the rabbits treatment,the acupuncture effect on the change including water,free radical,and calcium ion content of brain tissue were investigated.Results:Obvious cerebral edema and increasing free radical content occurred in cerebral schema group and repercussion group,the latter was the most serious( P <0 01).The increasing of calcium ion content in the early stage of ischemia(6h)was the most remarkable.Moreover,a notable positive correlation existed between cerebral and free radical(r=0.8076, P <0.05).Conclusion:Acupuncture treatment can notably improve the above indexes especially in the stage of repercussion.