小型猪低阻线区皮内微血管分布的研究

Study on the Distribution of Skin Microangium in the Low Impedance Line along Meridian of mini-Pig

目的：调查低阻线区皮内微血管的分布规律，为经络实质与微血管相关的观点提供形态学依据。方法：在确定了小型猪循经低阻线的基础上，随机切取长度５ｍ ｍ ～１０ ｍ ｍ ，宽度为３ ｍ ｍ ，深达肌肉或骨膜或腱的组织块，并以旁开５ｍ ｍ ～１０ ｍ ｍ 同样体积的组织块作对照，然后制成Ｈ．Ｅ染色的组织切片，并进行观察记数。结果：１．小型猪皮内微血管主要分布于真皮乳头层和皮下组织中，在乳头下层内的微血管网呈不均匀分布，多交织呈团块状，这种呈团块状的微血管网在本研究中被称作“微血管群”；２．经过１３９对组织块，３２８４２个视野的观察记数，在低阻线区与相对对照区的微血管群间，有９８组存在极显著差异，有４组存在显著差异，有３０组差异不显著，有１组存在显著负差异，有６组存在极显著负差异。以百分率作差异显著性检验，则Ｐ＜０．０１，表明小型猪低阻线区微血管群的丰富极有普遍性；３．经对９８对组织块的纵切片，１５１９９个视野内纵行微血管群数的观察计数，在实验组与相邻对照之间，有７０对存在极显著差异，有１８对存在显著差异，有９对差异不显著，有１对存在显著的负差异。以百分率作显著性检验，Ｐ＜ ０．０１，说明小型猪低阻线区皮内纵行微血管群的丰富极有普遍?

Objective:study on the distribution of skin microangiun in the low Impedance Line along Meridian LILM,in order to provide morphological foundation for the essence of meridian.Methods:Based on the identification of the LILM from mini-pig,a piece of tissue connected with tendon,muscle or bony membrane,was cut off randomly by 5 to 10 mm long and 3 mm wide,Meanwhile,another piece of tissue as its same size was also cut off as the control.Then,these tissue sections were stained with H.E and observed for amounting.Results:(1)The microangium in mini pig skin mainly distributed on stratum papilla,stratum hypo papilla and subcutaneous tissue.These microangium in stratum hypo papilla normally formal nets,but not distributed evenly.These dense microangium nets were called “microangium groups”in this paper.(2)According to the observation of 32842 times of 139 pairs of difference between the LILM microangium group and the control one( P <0.01).It showed that the plenty of microangium groups on LILM of mini pig were universal.(3)According to the observation of 15199 times of 98 pairs of vertical tissue piece on vertical microangium groups,there was a very significant difference between the experimental group and that control one( P <0.01).It showed that the plenty of vertically microangium groups on LILM of mini pig were universal.(4)According to the observation of 2580 times of 43 pairs of vertical tissue piece on those microangium linked with different microangium groups,there was a very significant difference between the experimental group and that control one( P <0.01).Conclusion:The microangium on LILM of mini pig were relatively plenty.They normally extended along LILM.Because of it,the convincing morphological foundation was provided for our hypothesis about the view point of “the essence of meridian being the ordered microangiun nets”