钩藤对自发性高血压大鼠心肌重构及原癌基因C-fos表达的影响

Experimental Study of Effects and Mechanisms of Rhynchophylla on Myocardial Remodeling and Expression of Proto-oncogene C-fos in Spontaneously Hypertensive Rat

目的 :观察钩藤煎剂浓缩液对自发性高血压大鼠 ( SHR)左室肥厚 ( LVH)及原癌基因 C- fos在心肌组织中表达的影响并初步探讨其机理。方法 :左室重 /体重比 ( LVW/BW)和透射电镜观察评价 LVH程度。免疫组化法测定左室心肌组织原癌基因 C- fos表达染色指数 ( SII)。结果 :钩藤组 SBP、LVW/BW、SII明显低于对照组 ,心肌超微结构基本恢复正常。结论 :钩藤能降低 SHR的 SBP,逆转 LVH,其作用机制可能与抑制原癌基因 C- fos表达有关。

Objective:To investigate the effects of rhynchophylla on hypertensive left ventricular(LVH)and expression of proto oncogene C fos in spontaneously hypertensive rat (SHR).Method:The level of LVH was assessed by means of left ventricle mass to body weight ratio(LVW/BW)and transmission electron microscope.The level of immunohistochemical stainning intensive indexes(SII)of C fos were anlayzed by immunohistochemical method.Results:The levels of SBP、LVW/BW and SII in SHRurh group were significantly lower than those of SHR control group.The myocardial urtrastructure of SHRurh group was regressed essentially Conclusions.The increasing of SBP of SHR can be inhibited obviously and LVH can be reversed by rhynchophylla,These processes of regression might related with the decreases of SBP and the inhibition of expression of proto oncogene C fos.