心气虚证动物模型的研制

Rsearch about the Animal Model of Heart Qi Deficiency

目的 :探索心气虚证动物模型的制作方法。方法 :80只 NIH小白鼠采用连续控食 2 4 d,每日强迫负重游泳 1 0 min,大剂量灌服心得安 (0 .5ml,1 mg/ml)和注射垂体后叶素 (0 .2 ml,5U/ml)等综合方法制造心气虚证动物模型。结果 :造模小鼠的临床表现与心气虚证诊断标准基本一致。心气虚证小鼠同时出现强迫负重游泳时间缩短 ,心率减慢 ,结代脉发生频率增高 ,心功能减退 ,超氧化物歧化酶 (SOD)活性降低 ,丙二醛(MDA)、乳酸脱氢酶 (LDH)和肌酸激酶 (CK)均值增大 ,与气虚组和对照组比较差别有显著性 (P<0 .0 5)或高度显著性 (P<0 .0 1 ) ,其中尤以心气虚 组各项检测变化更为明显。结论 :采用控食、惊劳。

To probe the methods of inducing the animal model of heart qi deficiency.Methods:The animal model of heart qi deficiency was induced by limiting the food continuously for 24 days,compelling to swim for 10 minutes per day,and giving a great quantity of propranolol (0.5 ml,1 mg/ml)and pituitrin (0.2 ml,5U/ml).Results:The symptoms and signs of the model rats were basically in correspondence with those of the patients with heart qi deficiency in clinic.The rats of heart qi dificiency indicated that the endurance of compelling to swim decreased,the heart beat become slow,the rate of bound and regularly interrupted pulse increased,the cardic function abated,myocardial SOD decreased MDA,LDH and CK incresed.There was significant or very significant difference compared with those of qi deficiency and control groups( P <0.05 or P <0.01).Heart qi deficiency group Ⅱ was more serious than heart qi deficiency group Ⅰ.Conclution:It is valuable to induce the animal model of heart qi deficiency by the multiple methods.