温补肾阳方对去卵巢所致骨质疏松大鼠IL-1和IL-6活性的影响

Effect of the Prescription for Warming and Recuperating the Kidney-Yang on the Activity of IL-1 and IL-6 in the Ovariectomy-induced Osteoporosis Rats

目的 :探讨温补肾阳方防治骨质疏松症的机理。方法 :以卵巢切除所致的骨质疏松大鼠为模型 ,采用骨组织形态计量学方法测定胫骨 TBV%、TRS%和 TFS% ,分别采用小鼠胸腺细胞检测法和依赖株细胞增殖反应检测法测定 IL- 1和 IL- 6的活性。结果 :温补肾阳方能使胫骨 TBV%显著增高 ,使 TRS%和TFS%均显著降低 ,并能使之降低到与正常大鼠相当的水平。大鼠切除卵巢后 ,IL- 1和 IL - 6活性显著增高 ,而温补肾阳方对这两者均有明显的抑制作用。结论 :温补肾阳方能够抑制 IL- 1、IL- 6的活性是其防治绝经后骨质疏松症的机理之一。

Objective:To study the mechanism of the prescription for warming and recuperating the Kidney-Yang preventing and treating the postmenopausal osteoporosis. Method: The ovariectomy-induced osteoporosis rat model was adopted. TBV%,TRS% and TFS% in shankbone were measured by bone histomorphometry. The activity of IL-1 was measured by the assay of the mouse's thymocyte. The activity of IL-6 was detected by the assay of proliferative response of dependent cell strain. Result: After the osteoporosis rats have been taken with the prescription for warming and recuperating the Kidney-Yang,TBV% heightened markedly,FRS% and TFS% in sharkbone reduced markedly. The activity of IL-1 and IL-6 increased markedly after rats have been spaied,but they can be inhibited with the prescription for warming and recuperating the Kidney-Yang. Conclusion: The Prescription for warming and recuperating the Kidney-Yang inhibiting the activity of IL-1 and IL-6 was one of mechanisms of its preventing and treating the postmenopausal osteoporosis.