肠梗阻对大鼠小肠、肝脏脂质过氧化损害的研究

Effect of intestinal obstruction on the small bowel and liver lipid peroxidization injury in rats

目的：探讨肠梗阻对大鼠小肠、肝脏的过氧化脂质损害作用。方法：复制大鼠小肠梗阻模型，检测了肠梗阻后４ｈ及解除梗阻后１ｈ血、小肠及肝脏组织中雨二醛含量，超氧化物歧化酶活性的变化。结果：肠梗阻后４ｈ，血清、小肠、肝脏组织中 ＭＤＡ含量显著升高，解除梗阻后１ｈＭＤＡ含量进一步升高（Ｐ＜０．０５）；肠梗阻后及解除梗阻后肝脏、小肠组织中ＳＯＤ活性下降，而全血ＳＯＤ活性增高（Ｐ＜０．０５）。结论：在肠梗阻状态下，自由基不仅直接导致肠组织的脂质过氧化损害，还可造成肝脏损害，这种损害在解除梗阻后１ｈ继续加重。

Objective To study the damage effect of intestinal obstruction on small bowel and liver lipid peroxidization. Methods The rats were made with acute small bowel obstruction as a model. One group (8rats)was obstructed for 4 hours, another group(8 rats)was obstructed and then removed the obstruction for 1 hours. One group(8 rats)was not obstructed as the control group. Superoxide dismutase (SOD) ac- tivity and the contents of Malondialdehybe (MDA) were detected in blood、liver and small bowel. Results MDA contents in the liver、 small bowel and serum were significantly increased after obstrurting for 4 hours, compared with control group(Ｐ<0.05). After removing obstruction, MDA contents futher increased(Ｐ<0.05). Activities of SOD in liver、small bowel tissue decreased(Ｐ<0.05). Nevertheless, activites of SOD in blood was increased. Conclusions The results indicate that oxygen - derived free radicals were involved in intestinal tissue damage under the obstruction. Free radicals not only result in lipid peroxidation of intestinal tissue, but also cause liver damage outside intestine.