半乳糖化白蛋白磁性阿霉素纳米粒对肝癌细胞株侵袭力的影响的实验研究

Experimental study on effect of Gal-HSA magnetic adriamycin nanoparticles on invasive ability of liver cancer cell line HepG2

目的研究半乳糖化白蛋白磁性阿霉素纳米粒对肝癌细胞株HepG2侵袭力的影响。方法应用半乳糖化白蛋白磁性阿霉素纳米粒于HepG2细胞,并设A组:RPMI-1640对照组、B组:普通盐酸阿霉素组(ADM)、C组:磁性白蛋白阿霉素纳米粒联合磁场(MADM+M)组、D组:半乳糖化白蛋白阿霉素纳米粒(Gal-ADM)组、E组:半乳糖化白蛋白磁性阿霉素纳米粒联合磁场(Gal-MADM+M)组。药物作用后以β-actin基因作为参照,半定量的逆转录-聚合酶链反应(RT-PCR)检测Cathepsin B-mRNA的表达差异,应用Transwell小室检测体外侵袭力的变化,并用裸鼠肝癌皮下转移模型检测对体内侵袭力的影响。结果半乳糖化白蛋白磁性阿霉素纳米粒作用HepG2肝癌细胞后Cathepsin B-mRNA表达降低较其他各组更为明显且有显著性,侵过小室滤膜细胞数目减少于其他各组且差异有显著性(P<0.01),裸鼠肝癌生长明显受到抑制(P<0.01)。结论半乳糖化白蛋白磁性阿霉素纳米粒抑制肝癌细胞株HepG2的侵袭力的作用较阿霉素、磁性白蛋白阿霉素纳米粒和半乳糖化白蛋白阿霉素纳米粒为强。

Objective: To study the effect of Gal-HSA magnetic adriamycin nanoparticles on invasive ability of liver cancer cell line HepG2. Methods: Cultured cells were divided into 5 groups: A: Control group, B: adriamycin (ADM) group; C: magnetic human serum albumin adriamycin nanoparticle combined magnetic field (MADM+M) group; D:galactosed human serum albumin adriamycin nanoparticles (Gal-ADM) group; E: galactosed magnetic human serum albumin adriamycin nanoparticle combined magnetic field (Gal-MADM+M) group. After application for 24, cultured cells were harvested. The difference of expression of Cathepsin B-mRNA were detected by the semi-quantitative reverse transcriptase polymerase chain reaction method (RT-PCR) with β-actin gene as reference. Invasive ability was measured by the number of cells to penetrate polocarbonates coated with matrigel. Human hepatocellular carcinoma transplanted subcutaneously in nude mice was constructed to detect the effect of Gal-HSA magnetic adriamycin nanoparticles on invasive ability in vivo. Results: Gal-HSA magnetic adriamycin nanoparticles could down-regulate the expression of of Cathepsin B-mRNA,and the inhibited ability was more effective than other group (P<0.01). The number of cells that penetrated polycarbonates was significantly lower in group than other group (P<0.01). Gal-HSA magnetic adriamycin nanoparticles could inhibit the growth of hepatocellular carcinoma transplanted in nake mice(P<0.01). Conclusion: Gal-HSA magnetic adriamycin nanoparticles can inhibit the invasive ability of liver cancer cells more effectively than adriamycin, magnetic HSA adriamycin nanoparticles and Gal-HSA adriamycin nanoparticles.