Effects of Zuogui Wan on neurocyte apoptosis and down-regulation of TGF-β_1 expression in nuclei of arcuate hypothalamus of monosodium glutamate-liver regeneration rats

AIM: To inquire into the effects and mechanism of Zuogui Wan (Pills for Kidney Yin) on neurocyte apoptosis in nuclei of arcuate hypothalamus (ARN) of monosodium glutamate (MSG)-Iiver regeneration rats, and the mechanism of liver regeneration by using optic microscope, electron microscope and in situ end labeling technology to adjust nerve-endocrine-immunity network.METHODS: Neurocyte apoptosis in ARN of the experiment rats was observed by using optic microscope, electron microscope and in situ end labeling technology. Expression of TGF-β1 in ARN was observed by using immunohistochemistry method.RESULTS: The expression of TGF-β1 in rats of model group was increased with the increase of ARN neurocyte apoptosis index (AI) (t = 8.3097, 12.9884, P<0.01). As compared with the rats of model group, the expression of TGF-β1 in rats of Zuogui Wan treatment group was decreased with the significant decrease of ARN neurocyte apoptosis (t = 4.5624, 11.1420, P<0.01).CONCLUSION: Brain neurocyte calcium ion overexertion and TGF-β1 protein participate in the adjustment and control of ARN neurocyte apoptosis in MSG-liver regeneration-rats. Zuogui Wan can prevent ARN neurocyte apoptosis of MSG-liver regeneration in rats by downregulating the expression of TGF-β1, and influence liver regeneration through adjusting nerve-endocrine-immunen etwork.

AIM:To inquire into the effects and mechanism of Zuogui Wan(Pills for Kidney Yin)on neurocyte apoptosis in nuclei of arcuate hypothalamus(ARN)of monosodium glutamate (MSG)-liver regeneration rats,and the mechanism of liver regeneration by using optic microscope,electron microscope and in situ end labeling technology to adjust nerve-endocrine- immunity network. METHODS:Neurocyte apoptosis in ARN of the experiment rats was observed by using optic microscope,electron microscope and in situ end labeling technology.Expression of TGF-β_1 in ARN was observed by using immunohistochemistry method. RESULTS:The expression of TGF-β_1 in rats of model group was increased with the increase of ARN neurocyte apoptosis index(AI)(t=8.3097,12.9884,P<0.01).As compared with the rats of model group,the expression of TGF-β_1 in rats of Zuogui Wan treatment group was decreased with the significant decrease of ARN neurocyte apoptosis(t=4.5624, 11.1420,P<0.01). CONCLUSION:Brain neurocyte calcium ion overexertion and TGF-β_1 protein participate in the adjustment and control of ARN neurocyte apoptosis in MSG-liver regeneration-rats.Zuogui Wan can prevent ARN neurocyte apoptosis of MSG-liver regeneration in rats by down- regulating the expression of TGF-β_1,and influence liver regeneration through adjusting nerve-endocrine-immune network.