摘要
AIM: To explore the activation of signal b-ansduction pathways related with the carcinogenesis of sporadic colon cancers. METHODS: A gene array monitoring the activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissues. The differentially expressed genes were further analyzed by RT-PCR, using RNA derived from colorectal cancer and normal colon tissue of 35 patients.RESULTS: The expression of HSF1, HSF27, HSP90 and iNOS was increased in colon cancer tissues compared to normal colon tissue using PathwayFinder GEArray. The RT-PCR results showed that the expression of HSF1 was increased in 86% (30/35) patients and the expression of iNOS was increased in 63% (22/35) patients.CONCLUSION: The induction of HSF1 gene expression is associated with sporadic colon cancer. HSF1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.
AIM:To explore the activation of signal transduction pathwaysrelated with the carcinogenesis of sporadic colon cancers.METHODS:A gene array monitoring the activation of 8 signaltransduction pathways(PathwayFinder GEArray)was usedto screen the differentially expressed genes betweencolorectal cancer and normal colon tissues.The differentiallyexpressed genes were further analyzed by RT-PCR,usingRNA derived from colorectal cancer and normal colon tissueof 35 patients.RESULTS:The expression of HSF1,HSF27,HSP90 and iNOSwas increased in colon cancer tissues compared to normalcolon tissue using PathwayFinder GEArray.The RT-PCRresults showed that the expression of HSF1 was increasedin 86%(30/35)patients and the expression of iNOS wasincreased in 63%(22/35)patients.CONCLUSION:The induction of HSF1 gene expression isassociated with sporadic colon cancer.HSF1 induces heatshock stress signaling pathway,which might play a role inthe carcinogenesis of sporadic colorectal cancer.
基金
Supported by 100 Scholars Plan of Chinese Academy of Sciences
