一种新型可用于DNA分子量标准的质粒构建

Construction of A New Kind of DNA Marker Vector

将首尾带有EcoRI酶切位点的 2 .0kb、1.5kb、1.0kb、0 .75kb、0 .5kb、0 .2 5kb六个长度的片段逐步连接到pGEM - 3zf(+)质粒上的BamHI位点中 ,构建的质粒用EcoRI进行单酶切 ,经电泳可以获得七条DNA带与设计结果完全一致 ,可用于DNA电泳试验中分子量标准。

Six fragments 2.0kb,1.5kb,1.0kb,0.75kb,0.5kb,0.25kb,which have two EcoR I restriction enzyme sites in each end side of them were recombined into BamH I restriction enzyme sites of pGEM-3zf(+) plasmid one by one.The restriction analysis demonstrated that the marker vector had been recombined.It can be use as DNA marker and will be very useful for the biotechnology lab.