家蚕浓核病毒中国株基因组DNA的分离纯化与鉴定

Purification and Identification of the Genome DNAs of Chinese Species of Bombyx mori.L Densovirus

家蚕浓核病毒中国株的正链DNA和负链DNA分别包裹在不同的病毒粒子中 ,而且两条链的大小不同 ,经高盐浓度的DNA抽提缓冲液提取后 ,在琼脂糖凝胶电泳谱中呈现出大小不同的两条带 ,一条 6 .4kb ,另一条 5 .8kb。作者分别用高盐和低盐抽提缓冲液提取了家蚕浓核病毒中国株的基因组DNA ,意外发现用低盐抽提缓冲液抽提出来的基因组DNA在琼脂糖凝胶电泳谱中仅呈现一条带。推测可能是由于大小不同且不完全互补的正负链 ,在低盐缓冲液中形成有效的互补 ,因而呈现一条 6 .2kb的条带。

The negetive DNA strain and the positive DNA strain of Chinese species of Bombyx mori densovirus(simply named Bm-DNV-Z in this article) are packaged to two different particles.The two DNA strains have different relative masses.So after electrophoresis in agarose gels.They display two different bands.Some research workers,not only in China,but also in other countries,thought that Bm-DNV-Z are the compouds of two different species of virus particles.In our research,we respectively used the low-salt abstraction buffer and the high-salt abstraction buffer to abstract genome DNAs of Bm-DNV-Z,surprisingly found that the genome DNAs abstracted by low-salt abstraction buffer displayed only one band about 6.2kb in the map of electrophoresis in 1% agarose gels,while the genome DNAs abstracted by high-salt abstraction buffer displayed two bands,one is 5.8kb,the other is 6.4kb.It implies there are maybe no virus particle of different species in Bm-DNV-Z,they are more like other Parvovirus,only have the negative and positive particles of the same species of virus.