摘要
A method was developed to quickly determine hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantiiby capillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, organic solvent, SDSconcentration and β-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separatedin 5 min. A good linear relationship between the peak area and concentration was found in the 6-500 μg/mL and 5-500 μg/mLconcentration range for hesperidin and naringin, respectively. The relative standard deviation based on migration time and peak area were0.82%、1.16% and 2.95%、3.08% for hesperidin and naringin, respectively. The detection limits based on three time noise were 1.2μg/mL and 1.5μg/mL for hesperidin and naringin, respectively. The method was verified by real sample analysis and runningstandard addition and recovery experiments with satisfactory results.
A method was developed to quickly determine hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantiiby capillary electrophoresis. Through the investigation of the effects of buffer pH and concentration, applied voltage, organic solvent, SDSconcentration and β-CD, the analytical conditions were optimized. Under the optimized conditions, the two analytes were well separatedin 5 min. A good linear relationship between the peak area and concentration was found in the 6-500 μg/mL and 5-500 μg/mLconcentration range for hesperidin and naringin, respectively. The relative standard deviation based on migration time and peak area were0.82%、1.16% and 2.95%、3.08% for hesperidin and naringin, respectively. The detection limits based on three time noise were 1.2μg/mL and 1.5μg/mL for hesperidin and naringin, respectively. The method was verified by real sample analysis and runningstandard addition and recovery experiments with satisfactory results.
出处
《生命科学仪器》
2004年第5期36-37,共2页
Life Science Instruments
基金
“十五”科技攻关重大项目(2001BA210A04-7)
国家自然科学基金(20175025)
国家自然科学基金分析化学重点基金(20235010)
电分析化学国家重点实验室基金
“九五”科技攻关重点项目(96-A23-01-06)的资助
