摘要
在1/2MS培养液内补料培养4周,人参发根R9923系中各单体皂苷含量与总皂苷含量大致上呈先降后升再降的过程,发根生物量与总皂苷含量呈上升趋势;不同瓶型培养的发根,其生物产量与皂苷含量随培养容积的增大而降低;发根工厂化培养适宜的接种量:种子罐为3～5g/50mL,培养2周,次级罐接种量为30g/400mL,再培养2周后,即可转入生产罐(5L)中继续培养至采收。
In 4-weeks' suspension culture total ginsenoside yielding and biomass of ginseng hairy root R9923 strain both showed increasing propensity, while ginsenoside contents and single saponin content showed a process of decreasing-increasing-decreasing; total ginsenoside yielding and biomass decreased with ginseng hairy root cultured in different volume of tank following the culture volume increased; seed volume in factory production was that 3～5g/50ml in a seed culture tank for 2 weeks, 30g/400ml in a secondary culture tank for 2 weeks and then transferred into productive tank till harvest.
出处
《特产研究》
2004年第3期11-15,共5页
Special Wild Economic Animal and Plant Research
基金
吉林省科学技术厅项目(20000203-2)
关键词
人参
发根诱导
R9923系
皂苷
组织培养
ginseng hairy root
variation of ginsenoside contents
production
