摘要
介绍了用测量~3H标记的底物被分解的量反映肠匀浆和血浆中二胺氧化酶活性的方法。条件实验结果表明,血浆和肠匀浆分别保温1～5h和0.25～5h,分解的~3H标记底物的dpm随保温时间延长而增高,不同稀释浓度的肠匀浆分解~3H标记的底物,也随稀释倍数增加而降低。取样后保存时间对酶活性影响较大,取样后,1d内可测得满意的结果。
A method is recommended for measuring diamine oxidase (DAO) activity based onthe determination of amount of ~3H-labelled substrate decomposed in intestinal homogenate andblood plasma. Experimental results under optimal conditions showed that dpm of ~3H-labelledsubstrate decomposed increased with the prolongation of incubation time when blood plasma andintestinal homogenate were incubated 1-5 hours and 0.25-5 hours respectively. The experimentalresults also showed that the decomposed ~3H-labelled substrate of intestinal homogenate diluted atdifferent concentrations decreased with increased dilution. The enzyme activity of DAO was great-ly affected by the time of preservation and satisfactory results were obtained within 1 day afterpreparation.
出处
《军事医学科学院院刊》
CSCD
北大核心
1993年第2期121-123,共3页
Bulletin of the Academy of Military Medical Sciences
关键词
二胺氧化酶
血浆
肠匀浆
diamine oxidase
plasma
intestinal homogenate
