冻存大鼠胚胎大脑组织的移植研究

Transplantation of fetal rat cerebral tissue after cryopreservation

对取自怀孕18d Wistar大鼠的胚胎鼠大脑组织进行冻存和移植研究的结果表明,当胚胎脑组织以1mol/L的DMSO作为低温保护剂,以1℃/min的降温速率降温至-70℃,在液氮中保存不同的时间后,37℃水浴快速复温并去除保护剂,然后移植到同种幼鼠的小脑实质内,12例移植物9例存活,移植物在受体脑内生长分化良好,具有正常的组织形态。

A study of cryopreservation and intracerebellar transplantation of embryonic cere-bral tissue of rats is reported. Prior to being cooled at a rate of 1℃ / min to -70℃, the tissueswere equilibrated in DMEM with 1mol / L dimethyl sulfoxide (DMSO). After storing in liquid ni-trogen for 1-6 months, the samples were thawed quickly in 37℃· water bath and the DMSO waseluted gradually. Then the freeze-thawed tissues were grafted into the cerebella of neonatal rats.The recipients survived for 40 to 80 days before their cerebella were processed by histologicalmethods. The results showed that nine of twelve transplants survived, well grown and differenti-ated within the host cerebella. The survival rates showed no obvious difference between the thawedtransplants and the fresh ones. From this and the previous studies on the same subject by others,we conclude that cryopreservation is a feasible and reliable method for storage of embryonic braintissue to be used later for transplantation.