核磁共振测定蛋白质构象

NMR investigation of protein conformation

核磁共振(NMR)已成为与X射线晶体衍射相补充的研究蛋白质构象的方法,而且其应用日益深入。本文综述了NMR测定蛋白质构象的基本原理、主要步骤、现状及前景,并对NMR的应用和成就与晶体衍射法进行了简要的比较讨论。

Protein conformation is one of the foundations of protein design and protein engi-neering. Nuclear magnetic resonance (NMR) spectroscopy is widely used in protein structure de-termination, in addition to the X ray diffraction techniques for single protein crystal. NMR meth-od enables one to carry out detailed structural study of protein in solution and non crystalline pro-teins and promises new insight into the dynamics of protein molecules and protein foldingproblem. This review briefly describes the strategies, procedures, current situation and prospect ofthe new approach.